PILRA Rat Monoclonal Antibody [Clone ID: 36H2]

CAT#: DDX0230A647-100

PILRA rat monoclonal antibody, clone 36H2

Product Images

WB analysis of treated-precipitates

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  IP of surface biotinylated monocytes lysates

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  Surface FACS staining of human PBMC

Specifications

Product Data

• Clone Name: 36H2
• Applications: FC, IF
• Recommended Dilution: DDX0230P-50 / DDX0230P-100 Purified: Surface Flow cytometry, Immunoprecipitation, Western Blot.
  DDX0230A488-50 / DDX0230A488-100 Alexa-fluor®488: Surface Flow cytometry, ImmunoFluorescence.
  DDX0230A546-50 / DDX0230A546-100 Alexa-fluor®488: Surface Flow cytometry, ImmunoFluorescence.
  DDX0230A647-50 / DDX0230A647-100 Alexa-fluor®488: Surface Flow cytometry, ImmunoFluorescence.
  DDX0230B-50 / DDX0230B-100 Biotin: Western Blot.
  
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Human
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant FDF03-Ig fusion protein.
• Specificity: Human FDF03.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 647
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 647
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Background: Human FDF03, is a novel member of the Ig superfamily expressed as a monomeric 44 kDa transmembrane glycoprotein and containing a single extracellular V-set Ig-like domain. Two potential secreted isoforms were also identified. FDF03 was mostly detected in hemopoietic tissues and was expressed by monocytes, macrophages, and granulocytes, but not by lymphocytes (B, T, and NK cells), indicating an expression restricted to the myelomonocytic lineage. FDF03 was also strongly expressed by monocyte-derived dendritic cells (moDC) and preferentially by CD14+/CD1a- DC derived from CD34+ progenitors. Moreover, flow cytometric analysis showed FDF03 expression by CD11c+ blood and tonsil DC, but not by CD11c- DC precursors. The FDF03 cytoplasmic tail contained two immunoreceptor tyrosine-based inhibitory motifs (ITIM)-like sequences. Human FDF03 is a novel ITIM-bearing receptor selectively expressed by cells of myeloid origin, including DC, which may regulate functions other than that of the broadly distributed LAIR-1/p40 molecule. (Fournier N et al, 2000; J. Immunol., 165(3):1197-1209).
• Synonyms: PILR-alpha, FDF03