IGSF6 Mouse Monoclonal Antibody [Clone ID: 104A10.01]

CAT#: DDX0220A546-100

IGSF6 mouse monoclonal antibody, clone 104A10.01

Product Images

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  staining of murine DORA – transfected COP5 cells

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  FACS staining of humanDORA – transfected COP5 cells

Specifications

Product Data

• Clone Name: 104A10.01
• Applications: FC, WB
• Recommended Dilution: Flow Cytometry surface, Western Blot.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-15 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use.
  
• Reactivities: Canine, Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant DORA Ig fusion protein.
• Specificity: Human DORA.
  Species cross-reactivity: Mouse, Canine.
• Formulation: Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
  Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
  Label: Alexa Fluor 546
• Concentration: lot specific
• Purification: QMA Hyper D ion exchange chromatography
• Conjugation: Alexa Fluor 546
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Gene Name: immunoglobulin superfamily member 6
• Database Link:
  Entrez Gene 10261 Human
  UniProt ID O95976
• Background: Using a cDNA subtraction technique, a novel member of the immunoglobulin superfamily was isolated from human dendritic cells (DCs). This cDNA, named DORA, for Down-Regulated by Activation encodes a protein belonging to the CD8 family of receptors containing a single V type loop domain with an associated J chain region, a transmembrane region containing an atypical tyrosine residue and a cytoplasmic domain containing three putative tyrosine phosphorylation sites. The human DORA gene has been mapped to chromosome 16. Expression is observed in DCs, purified ex vivo or generated in vitro from either monocytes or CD34+ progenitors, and down-regulated following activation by PMA and Ionomycin or by CD40L engagement.
  (Bates EE et al, 2000; Immunogenetics, 52: 112-20; Bates EE et al, 1998; Mol. Immunology, 35: 513-24).
• Synonyms: DORA