BETVIA Human Monoclonal Antibody [Clone ID: H.G5F2A4.09]

CAT#: DDX0074P-100

BETVIA human monoclonal antibody, clone H.G5F2A4.09

Product Images

Enriched basophils from birch pollen were incubated with concentrations of nBetV1 inducing from 50 to 100% of the maximal histamine release in the presence or the absence of the mentioned HumAbs. The histamine releasewas expressed as a percentage of maximal histamine release. The mean and SD of three measurements are presented. A, when tested indicidually, only BAB1 inhibits the Betv1-induced histamine release. The five HumAbs were individually preincubated, at concentrations ranging from 0.4 to 1.5mg/ml with nBetv1 before challenging exposure of the basophils of two allergic patients (M.M and J.C). B, BAB 1 preincubated at 1.5mg/ml with nBetv1 inhibits the histamine release from the basophils of the four patients tested (H.L, E.B, M.M, and J.C). C, the Betv1-induced histamine release inhibition is specific and dose-dependent. nBetv1 was preincubated with successive dilutions of an anti-IL1a HumAB X3A3 (starting from 1.3mg/ml), of BAB1 (starting from 1.5mg/ml) or of amixture of BAB2 to BAB5 (starting at 0.1mg/ml for each) and then exposed to the baophils from patient J.C.

Specifications

Product Data

• Clone Name: H.G5F2A4.09
• Applications: ELISA, WB
• Recommended Dilution: ELISA, histamine release blocking , WB
  Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
• Reactivities: Pollen
• Host: Human
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: human B cells immortalized from a birch pollen allergic patient
• Specificity: birch pollen Bet v1
  Cross-reactivity: large panel of tree’s pollen
• Formulation: State: Purified
  State: 100 μg in 200 μl Tris-NaCl pH 8
• Concentration: lot specific
• Purification: QMA Hyper-D Ion-exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Background: Birch pollen allergy is a frequent pathology with IgE reactivity against Betv1 the major birch pollen allergen. Human monoclonal antibodies secreting B cells have been cloned from PBMC of allergic patients. These high affinity human monoclonal antibodies raised against BetV1 inhibit the binding of IgE to nitrocellulose-blotted Bet v1 and inhibit histamine release by sensitized basophils. (Visco V. et al, J. Immunol., 1996 ; 157: 956-962 ; Denépoux S et al, FEBS Lett. 2000 Jan 7;465(1):39-46.)
• Synonyms: BETVI, Allergen Bet v I-A