BETVIA Human Monoclonal Antibody [Clone ID: H.G28C10.01]

Enriched basophils from birch pollen were incubated with concentrations of nBetV1 inducing from 50 to 100% of the maximal histamine release in the presence or the absence of the mentioned HumAbs. The histamine releasewas expressed as a percentage of maximal histamine release. The mean and SD of three measurements are presented. A, when tested indicidually, only BAB1 inhibits the Betv1-induced histamine release. The five HumAbs were individually preincubated, at concentrations ranging from 0.4 to 1.5mg/ml with nBetv1 before challenging exposure of the basophils of two allergic patients (M.M and J.C). B, BAB 1 preincubated at 1.5mg/ml with nBetv1 inhibits the histamine release from the basophils of the four patients tested (H.L, E.B, M.M, and J.C). C, the Betv1-induced histamine release inhibition is specific and dose-dependent. nBetv1 was preincubated with successive dilutions of an anti-IL1a HumAB X3A3 (starting from 1.3mg/ml), of BAB1 (starting from 1.5mg/ml) or of amixture of BAB2 to BAB5 (starting at 0.1mg/ml for each) and then exposed to the baophils from patient J.C.

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SKU

DDX0073P-100

BETVIA human monoclonal antibody, clone H.G28C10.01

Datasheet

$1,050.00

2 Weeks*

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Specifications

Specifications
Product Data
Clone Name	H.G28C10.01
Application	ELISA
Recommended Dilution	ELISA (coating), IP. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml.
Reactivity	Pollen
Antibody Host	Human
Isotype	IgG4
Clonality	Monoclonal
Immunogen	human B cells immortalized from a birch pollen allergic patient
Specificity	birch pollen Bet v1 Cross-reactivity: large panel of tree’s pollen.
Buffer	State: Purified State: 100 μg in 200 μl Tris-NaCl pH 8
Concentration	lot specific
Purification	QMA Hyper-D Ion-exchange chromatography
Conjugation	Unconjugated
Storage	-20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing.
Shipping	Blue Ice
Background	Birch pollen allergy is a frequent pathology with IgE reactivity against Betv1 the major birch pollen allergen. Human monoclonal antibodies secreting B cells have been cloned from PBMC of allergic patients. These high affinity human monoclonal antibodies raised against BetV1 inhibit the binding of IgE to nitrocellulose-blotted Bet v1 and inhibit histamine release by sensitized basophils. (Visco V. et al, J. Immunol., 1996 ; 157: 956-962 ; Denépoux S et al, FEBS Lett. 2000 Jan 7;465(1):39-46.)
Synonyms	BETVI, Allergen Bet v I-A
Reference Data

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Documents

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard

Resources

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

BETVIA Human Monoclonal Antibody [Clone ID: H.G28C10.01]

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Citations