Birch Pollen (allergen Bet v1) Mouse Monoclonal Antibody [Clone ID: 102H10.05]

CAT#: DDX0050P-100

Birch Pollen (allergen Bet v1) mouse monoclonal antibody, clone 102H10.05

Product Images

Cross-reactivity with Bet v 1 homologous plant allergens

• 

  mAbs binding to nitrocellulose-blotted natural (n) or recombinant birchpollen Bet v 1

• 

  Modulation of IgE (allergic patients) binding to Bet v 1

Specifications

Product Data

• Clone Name: 102H10.05
• Applications: Neutralize
• Recommended Dilution: Binding to nitrocellulose-blotted Bet v 1, inhibition of human IgE binding to Bet v1, cross-competition with anti-Bet v1 mAbs.
  Usage recommendation:
  *This monoclonal antibody may be used between 5-20 μg/ml.
  *Optimal dilution should be determined by each laboratory for each application.
  *Coupled antibody: to maintain RT before use
  
• Reactivities: Pollen
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: pollen grains from Betula alba
• Specificity: Birch pollen Bet v 1
  Cross-reactivity: Bet v 1 homologous plant allergens.
• Formulation: State: Purified
  State: 100 μg in 200 μl Tris-NaCl pH 8
• Concentration: lot specific
• Purification: QMA Hyper-D Ion-exchange chromatography
• Conjugation: Unconjugated
• Storage: -20°C. KEEP CONTENTS STERILE: no preservative.
  Purified antibodies: avoid repeated freeze/thaw cycles.
  Coupled antibodies: glycerol protects from freezing.
• Background: Bet v 1 and homologous proteins represent major allergens for almost 95% of patients allergic to tree pollen and approximately 70% of those allergic to fruits and vegetables. Birch pollen allergy is a frequent pathology with IgE reactivity against Bet v 1. A panel of 85 mouse monoclonal anti-Bet v 1 antibodies was raised as a tool to study the interaction of human IgE antibodies with Bet v 1. Cross-reactivities with Bet v 1 homologous plant allergens, modulation of IgE binding to Bet v 1, Bet v 1 immunopurification, and inhibition of histamine release are described in the original article.
  (Lebecque S et l, J Allergy Clin Immunol, 1997; 99:374-84).