For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation
Unconjugated
Storage
Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
Stability
Stable for 12 months from date of receipt.
Shipping
Ambient
Predicted Protein Size
70KD
Background
AAV vectors are promising delivery tools for human gene therapy. AAV is a single-stranded DNA parvovirus with a 4.7 kb genome composed of the rep and cap genes flanked by inverted terminal repeats (ITRs). The rep gene encodes non-structural proteins involved in viral replication, packaging, and genomic integration, whereas the cap gene codes for structural proteins (VP1, VP2, VP3) that assemble to form the viral capsid, which serves as the viral gene delivery vehicle.
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