TIGIT Mouse Monoclonal Antibody [Clone ID: OTI7E5]

CAT#: CF813031

Carrier-free (BSA/glycerol-free) TIGIT mouse monoclonal antibody,clone OTI7E5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIGIT (RC221447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIGIT (1:1000).

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  Flow cytometric analysis of living 293T cells transfected with TIGIT overexpression plasmid (RC221447), Red)/empty vector (PS100001, Blue) using anti-TIGIT antibody (TA813031). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI7E5
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TIGIT (NP_776160) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.1 kDa
• Gene Name: Homo sapiens T cell immunoreceptor with Ig and ITIM domains (TIGIT), mRNA.
• Database Link:
  NP_776160
  Entrez Gene 201633 Human
  UniProt ID Q495A1
• Background: This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [provided by RefSeq, Sep 2009]
• Synonyms: VSIG9; VSTM3; WUCAM

Reference Data

• Protein Families: Transmembrane