B7-1 (CD80) Mouse Monoclonal Antibody [Clone ID: OTI11G5]

CAT#: CF812246

Carrier-free (BSA/glycerol-free) CD80 mouse monoclonal antibody,clone OTI11G5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (RC206540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80 (1:500).

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  Western blot analysis of extracts (35ug) from cell lines and/or tissue lysates by using anti-CD80 monoclonal antibody (1:500).

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  CD80 ELISA with 7D6 (TA812242) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

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  CD80 ELISA with 1G9 (TA812243) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

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  CD80 ELISA with 1A10 (TA812244) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

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  HEK293T cells transfected with either RC206540 overexpress plasmid (Red) or IgG isotype control (Blue) were immunostained by anti-CD80 antibody (TA812246), and then analyzed by flow cytometry (1:100).

• Flow cytometric Analysis of living Jurkat cells, using anti-CD80 antibody (TA812246), (Red), compared to an IgG isotype control, (Blue) (1:100).

• Flow cytometric Analysis of living Raji cells, using anti-CD80 antibody (TA812246), (Red), compared to an IgG isotype control, (Blue) (1:100).

• CD80 Luminex with 7D6 (TA812242) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

• CD80 Luminex with 1G9 (TA812243) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

• CD80 Luminex with 1A10 (TA812244) Capture and 11G5 (TA812246) Detection Antibodies. Substrate used: Recombinant Human CD80 (TP700242)

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Specifications

Product Data

• Clone Name: OTI11G5
• Applications: ELISA, FC, LMNX, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD80 molecule
• Database Link:
  NP_005182
  Entrez Gene 941 Human
  UniProt ID P33681
• Background: The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
• Synonyms: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis