Repulsive Guidance Molecule A (RGMA) Mouse Monoclonal Antibody [Clone ID: OTI1C5]

CAT#: CF811571

Carrier-free (BSA/glycerol-free) RGMA mouse monoclonal antibody,clone OTI1C5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RGMA (Cat# RC221996, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RGMA (Cat# TA811571)(1:2000). Positive lysates LY402781 (100ug) and LC402781 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1C5
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 169-424 of human RGMA (NP_064596) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.2 kDa
• Gene Name: repulsive guidance molecule BMP co-receptor a
• Database Link:
  NP_064596
  Entrez Gene 244058 MouseEntrez Gene 308739 RatEntrez Gene 56963 Human
  UniProt ID Q96B86
• Synonyms: RGM