Nuclear Factor 1 (NFIA) Mouse Monoclonal Antibody [Clone ID: OTI4G8]

CAT#: CF811215

Carrier-free (BSA/glycerol-free) NFIA mouse monoclonal antibody,clone OTI4G8

Product images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NFIA (RC225878, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NFIA (1:2000). Positive lysates LY427477 (100ug) and LC427477 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4G8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivity: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 200-509 of human NFIA (NP_001128145) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.8 kDa
• Gene Name: Homo sapiens nuclear factor I A (NFIA), transcript variant 1, mRNA.
• Database Link:
  NP_001128145
  Entrez Gene 18027 MouseEntrez Gene 25492 RatEntrez Gene 4774 Human
• Synonyms: CTF; NF-I/A; NF1-A; NFI-A; NFI-L; BRMUTD

Reference Data

• Protein Families: Transcription Factors