RNF149 Mouse Monoclonal Antibody [Clone ID: OTI8D7]

CAT#: CF810580

Carrier-free (BSA/glycerol-free) RNF149 mouse monoclonal antibody,clone OTI8D7

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF149 (RC207979, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF14. Positive lysates LY406531 (100ug) and LC406531 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 5 cell lines by using anti-RNF149 monoclonal antibody. (1:2000) (1:500)

Specifications

Product Data

• Clone Name: OTI8D7
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 250-383 of human RNF149 (NP_775918) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: Homo sapiens ring finger protein 149 (RNF149), mRNA.
• Database Link:
  NP_775918
  Entrez Gene 284996 Human
  UniProt ID Q8NC42
• Synonyms: DNAPTP2

Reference Data

• Protein Families: Druggable Genome, Transmembrane