TRMT12 Mouse Monoclonal Antibody [Clone ID: OTI3D2]

CAT#: CF809540

Carrier-free (BSA/glycerol-free) TRMT12 mouse monoclonal antibody,clone OTI3D2

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT12 (RC203982, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT12 (1:2000). Positive lysates LY402634 (100ug) and LC402634 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3D2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TRMT12 (NP_060426) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.1 kDa
• Gene Name: tRNA methyltransferase 12 homolog
• Database Link:
  NP_060426
  Entrez Gene 55039 Human
  UniProt ID Q53H54
• Background: Wybutosine (yW) is a hypermodified guanosine at the 3-prime position adjacent to the anticodon of phenylalanine tRNA that stabilizes codon-anticodon interactions during decoding on the ribosome. TRMT12 is the human homolog of a yeast gene essential for yW synthesis (Noma and Suzuki, 2006). [supplied by OMIM, Mar 2008]. COMPLETENESS: complete on the 3' end.
• Synonyms: TRM12; TYW2