UCK2 Mouse Monoclonal Antibody [Clone ID: OTI21A6]

CAT#: CF809419

Carrier-free (BSA/glycerol-free) UCK2 mouse monoclonal antibody,clone OTI21A6

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY UCK2 (Cat# RC202406, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-UCK2 (Cat# TA809419)(1:500). Positive lysates LY402221 (100ug) and LC402221 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI21A6
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to residues near C-terminus of human UCK2 (NP_036606).
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.1 kDa
• Gene Name: uridine-cytidine kinase 2
• Database Link:
  NP_036606
  Entrez Gene 7371 Human
  UniProt ID Q9BZX2
• Background: This gene encodes a pyrimidine ribonucleoside kinase. The encoded protein (EC 2.7.1.48) catalyzes phosphorylation of uridine and cytidine to uridine monophosphate (UMP) and cytidine monophosphate (CMP), respectively. [provided by RefSeq, Oct 2010]
• Synonyms: TSA903; UK; UMPK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Pyrimidine metabolism