ELP4 Mouse Monoclonal Antibody [Clone ID: OTI4A9]

CAT#: CF809233

Carrier-free (BSA/glycerol-free) ELP4 mouse monoclonal antibody,clone OTI4A9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ELP4 (RC219128, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ELP4 (1:2000). Positive lysates LY412806 (100ug) and LC412806 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4A9
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 133-407 of human ELP4 (NP_061913) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens elongator acetyltransferase complex subunit 4 (ELP4), transcript variant 1, mRNA.
• Database Link:
  NP_061913
  Entrez Gene 26610 Human
  UniProt ID Q96EB1
• Background: This gene encodes a component of the six subunit elongator complex, a histone acetyltransferase complex that associates directly with RNA polymerase II during transcriptional elongation. The human gene can partially complement sensitivity phenotypes of yeast ELP4 deletion mutants. This gene has also been associated with Rolandic epilepsy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
• Synonyms: AN; C11orf19; dJ68P15A.1; hELP4; PAX6NEB; PAXNEB