NAPSIN A (NAPSA) Mouse Monoclonal Antibody [Clone ID: OTI3A4]

CAT#: CF808773

Carrier-free (BSA/glycerol-free) NAPSA mouse monoclonal antibody, clone OTI3A4

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808773) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808773) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NAPSA mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808773) (1:150)

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  Immunocytochemistry staining of A549 cells using anti-NAPSA mouse monoclonal antibody (TA808773). The right is HELA cells as negative control.

Specifications

Product Data

• Clone Name: OTI3A4
• Applications: IF, IHC
• Recommended Dilution: IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 64-244 of human NAPSA(NP_004842) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42.7 kDa
• Gene Name: Homo sapiens napsin A aspartic peptidase (NAPSA), mRNA.
• Database Link:
  NP_004842
  Entrez Gene 9476 Human
  UniProt ID O96009
• Background: The activation peptides of aspartic proteinases plays role as inhibitors of the active site. These peptide segments, or pro-parts, are deemed important for correct folding, targeting, and control of the activation of aspartic proteinase zymogens. The pronapsin A gene is expressed predominantly in lung and kidney. Its translation product is predicted to be a fully functional, glycosylated aspartic proteinase precursor containing an RGD motif and an additional 18 residues at its C-terminus. [provided by RefSeq, Jul 2008]
• Synonyms: KAP; Kdap; NAP1; NAPA; SNAPA

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome