LGR5 Mouse Monoclonal Antibody [Clone ID: OTI3F1]

CAT#: CF808748

Carrier-free (BSA/glycerol-free) LGR5 mouse monoclonal antibody, clone OTI3F1 (formerly 3F1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR5 (RC212825, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR5 (1:2000). Positive lysates LY401213 (100ug) and LC401213 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of stable transfection cell line LGR5 (RC212825) (Red) compared to negative control HEK293T cell line (blue)using anti-LGR5 antibody (TA808748) (1:50)

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  Flow cytometric analysis of the stable expression of LGR5 (RC212825) in NIH3T3 cells using anti-LGR5 antibody (TA808748) (Red) compared to a nonspecific negative control antibody (Black). (1:100), surface stained on live cells

Specifications

Product Data

• Clone Name: OTI3F1
• Applications: FC, WB
• Recommended Dilution: FLOW 1:50~100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 22-543 of human LGR5 (NP_003658) produced in HEK293 cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 99.8 kDa
• Gene Name: Homo sapiens leucine rich repeat containing G protein-coupled receptor 5 (LGR5), transcript variant 1, mRNA.
• Database Link:
  NP_003658
  Entrez Gene 14160 MouseEntrez Gene 299802 RatEntrez Gene 8549 Human
  UniProt ID O75473
• Synonyms: FEX; GPR49; GPR67; GRP49; HG38

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane