ERCC8 Mouse Monoclonal Antibody [Clone ID: OTI5C9]

CAT#: CF808545

Carrier-free (BSA/glycerol-free) ERCC8 mouse monoclonal antibody,clone OTI5C9

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC8 (RC203124, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC8 (1:500). Positive lysates LY423459 (100ug) and LC423459 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue within the normal limits using anti-ERCC8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808545) (1:150)

Specifications

Product Data

• Clone Name: OTI5C9
• Applications: IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERCC8(NP_001007235) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43.9 kDa
• Gene Name: Homo sapiens ERCC excision repair 8, CSA ubiquitin ligase complex subunit (ERCC8), transcript variant 3, mRNA.
• Database Link:
  NP_001007235
  Entrez Gene 71991 MouseEntrez Gene 310071 RatEntrez Gene 1161 Human
  UniProt ID Q13216
• Background: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
• Synonyms: CKN1; CSA; UVSS2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis