ERCC8 Mouse Monoclonal Antibody [Clone ID: OTI2G1]

SKU CF808524

Carrier-free (BSA/glycerol-free) ERCC8 mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)

Product Data

• Clone Name: OTI2G1
• Application: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivity: Human, Mouse, Rat
• Antibody Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERCC8(NP_001007235) produced in E.coli.
• Buffer: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Ambient
• Predicted Protein Size: 43.9 kDa
• Gene Name: ERCC excision repair 8, CSA ubiquitin ligase complex subunit
• Database Link:
  NP_001007235
  Q13216
• Background: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
• Synonyms: CKN1; CSA; UVSS2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis