BTG2 Mouse Monoclonal Antibody [Clone ID: OTI1G3]

CAT#: CF808149

Carrier-free (BSA/glycerol-free) BTG2 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTG2 (RC214600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTG2 (1:2000). Positive lysates LY402022 (100ug) and LC402022 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA808149) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BTG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA808149) (1:150)

Specifications

Product Data

• Clone Name: OTI1G3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTG2 (NP_006754) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.2 kDa
• Gene Name: Homo sapiens BTG anti-proliferation factor 2 (BTG2), mRNA.
• Database Link:
  NP_006754
  Entrez Gene 12227 MouseEntrez Gene 29619 RatEntrez Gene 7832 Human
  UniProt ID P78543
• Background: The protein encoded by this gene is a member of the BTG/Tob family. This family has structurally related proteins that appear to have antiproliferative properties. This encoded protein is involved in the regulation of the G1/S transition of the cell cycle. [provided by RefSeq, Jul 2008]
• Synonyms: APRO1; PC3; TIS21

Reference Data

• Protein Families: Transcription Factors