APPL (APPL1) Mouse Monoclonal Antibody [Clone ID: OTI7C3]

CAT#: CF807767

Carrier-free (BSA/glycerol-free) APPL1 mouse monoclonal antibody,clone OTI7C3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (RC206021, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APPL1 (1:500). Positive lysates LY402145 (100ug) and LC402145 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI7C3
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human APPL1 (NP_036228) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
• Database Link:
  NP_036228
  Entrez Gene 72993 MouseEntrez Gene 290537 RatEntrez Gene 26060 Human
  UniProt ID Q9UKG1
• Background: The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]
• Synonyms: APPL; DIP13alpha; MODY14

Reference Data

• Protein Pathways: Colorectal cancer, Pathways in cancer