GPNMB Mouse Monoclonal Antibody [Clone ID: OTI8A10]

CAT#: CF807721

Carrier-free (BSA/glycerol-free) GPNMB mouse monoclonal antibody, clone OTI8A10 (formerly 8A10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GPNMB (Cat# RC207615, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GPNMB(Cat# TA807721). Positive lysates LY400379 (100ug) and LC400379 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GPNMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807721)

• 

  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-GPNMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA807721)

Specifications

Product Data

• Clone Name: OTI8A10
• Applications: IHC, WB
• Recommended Dilution: WB 1:200~2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GPNMB (NP_001005340) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: glycoprotein nmb
• Database Link:
  NP_001005340
  Entrez Gene 10457 Human
  UniProt ID Q14956
• Background: The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: HGFIN; NMB

Reference Data

• Protein Families: Druggable Genome, Transmembrane