LOXL2 Mouse Monoclonal Antibody [Clone ID: OTI2A7]

CAT#: CF807441

Carrier-free (BSA/glycerol-free) LOXL2 mouse monoclonal antibody, clone OTI2A7 (formerly 2A7)

Product Images

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and LOXL2-Knockout hela cells (KO, Cat# LC810132) were separated by SDS-PAGE and immunoblotted with anti-LOXL2 monoclonal antibody TA807441. Then the blotted membrane was stripped and reprobed with anti-β-actin (TA811000) as a loading control (1:500).

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LOXL2 (RC200455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LOXL2. Positive lysates LY400844 (100ug) and LC400844 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A7
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 152-450 of human LOXL2(NP_002309) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84 kDa
• Gene Name: Homo sapiens lysyl oxidase like 2 (LOXL2), mRNA.
• Database Link:
  NP_002309
  Entrez Gene 94352 MouseEntrez Gene 290350 RatEntrez Gene 4017 Human
  UniProt ID Q9Y4K0
• Background: This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]
• Synonyms: LOR2; WS9-14

Reference Data

• Protein Families: Druggable Genome, Secreted Protein