CD3E Mouse Monoclonal Antibody [Clone ID: UCHT1]

CAT#: CF807198

Carrier-free (BSA/glycerol-free) CD3 mouse monoclonal antibody, clone UCHT1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD3 (RC208276, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD3 (1:2000). Positive lysates LY400242 (100ug) and LC400242 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of live Jurkat cells, using anti-CD3 antibody (TA807198), (Red), compared to a nonspecific negative control antibody (Mouse-IGg), (Blue) (1:100).

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  Flow cytometric Analysis of living RBC-lysed human peripheral blood cells, using anti-CD3 antibody (TA807198, Red), compared to an IgG isotype control (green), and negative control (PBS, Blue) (1:100).

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  Flow cytometric Analysis of living peripheral blood mononuclear cells (PBMC), using anti-CD3 antibody (TA807198, Red), compared to an IgG isotype control (Blank) (1:100).

Specifications

Product Data

• Clone Name: UCHT1
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human infant thymocytes and lymphocytes from a patient with Sézary disease
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.7 kDa
• Gene Name: CD3e molecule
• Database Link:
  NP_000724
  Entrez Gene 916 Human
  UniProt ID P07766
• Background: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
• Synonyms: IMD18; T3E; TCRE

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway