SPC25 Mouse Monoclonal Antibody [Clone ID: OTI1F8]

CAT#: CF806562

Carrier-free (BSA/glycerol-free) SPC25 mouse monoclonal antibody, clone OTI1F8 (formerly 1F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPC25 (RC204623, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPC25. Positive lysates LY412309 (100ug) and LC412309 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1F8
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SPC25 (NP_065726) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26 kDa
• Gene Name: SPC25 component of NDC80 kinetochore complex
• Database Link:
  NP_065726
  Entrez Gene 57405 Human
  UniProt ID Q9HBM1
• Background: This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity. [provided by RefSeq, Jul 2008]
• Synonyms: AD024; hSpc25; SPBC25

Reference Data

• Protein Families: Druggable Genome