Neurofilament (NEFM) Mouse Monoclonal Antibody [Clone ID: OTI2G3]

CAT#: CF805823

Carrier-free (BSA/glycerol-free) NEFM mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEFM (RC224475, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEFM. Positive lysates LY417338 (100ug) and LC417338 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and NEFM-Knockout 293T cells (KO, Cat# LC810526) were separated by SDS-PAGE and immunoblotted with anti-NEFM monoclonal antibody TA805823, (1:5000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human skin tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

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  Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

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  Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

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  Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

• Immunohistochemical staining of paraffin-embedded Human embryonic liver tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

• Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

• Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

• Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

• Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-NEFM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA805823)

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Specifications

Product Data

• Clone Name: OTI2G3
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 413-773 of human NEFM(NP_005373) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 102.3 kDa
• Gene Name: neurofilament medium chain
• Database Link:
  NP_005373
  Entrez Gene 4741 Human
  UniProt ID P07197
• Background: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
• Synonyms: NEF3; NF-M; NFM

Reference Data

• Protein Pathways: Amyotrophic lateral sclerosis (ALS)