Neurofilament (NEFM) Mouse Monoclonal Antibody [Clone ID: OTI2G3]

CAT#: CF805823

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Carrier-free (BSA/glycerol-free) NEFM mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Formulation: Standard Carrier-Free



  View other "OTI2G3" antibodies (4)

USD 600.00

In Stock*

Size
    • 100 ug

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Specifications

Product Data
Clone Name OTI2G3
Applications IHC, WB
Recommended Dilution WB 1:2000, IHC 1:150
Reactivities Human
Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Human recombinant protein fragment corresponding to amino acids 413-773 of human NEFM(NP_005373) produced in E.coli.
Formulation Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
Reconstitution Method For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
Purification Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Predicted Protein Size 102.3 kDa
Gene Name Homo sapiens neurofilament medium chain (NEFM), transcript variant 1, mRNA.
Background Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Synonyms NEF3; NF-M; NFM
Reference Data
Protein Pathways Amyotrophic lateral sclerosis (ALS)

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