Calpain 5 (CAPN5) Mouse Monoclonal Antibody [Clone ID: OTI4E11]

CAT#: CF804908

Carrier-free (BSA/glycerol-free) CAPN5 mouse monoclonal antibody, clone OTI4E11 (formerly 4E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPN5 (RC202045, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPN5. Positive lysates LY418247 (100ug) and LC418247 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4E11
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 274-578 of human CAPN5 (NP_004046) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73 kDa
• Gene Name: calpain 5
• Database Link:
  NP_004046
  Entrez Gene 12337 MouseEntrez Gene 171495 RatEntrez Gene 726 Human
  UniProt ID O15484
• Background: Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. [provided by RefSeq, Jul 2008]
• Synonyms: ADNIV; HTRA3; nCL-3; VRNI

Reference Data

• Protein Families: Druggable Genome, Protease