IGSF11 Mouse Monoclonal Antibody [Clone ID: OTI3A4]

CAT#: CF803775

Carrier-free (BSA/glycerol-free) IGSF11 mouse monoclonal antibody, clone OTI3A4 (formerly 3A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGSF11 (RC206735, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGSF11. Positive lysates LY407451 (100ug) and LC407451 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGSF11 mouse monoclonal antibody. (TA803775) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IGSF11 mouse monoclonal antibody. (TA803775) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IGSF11 mouse monoclonal antibody. (TA803775) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGSF11 mouse monoclonal antibody. (TA803775) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-IGSF11 mouse monoclonal antibody. (TA803775) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-IGSF11 mouse monoclonal antibody. (TA803775) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-IGSF11 mouse monoclonal antibody. (TA803775) Dilution: 1:150

Show all 8 images

Specifications

Product Data

• Clone Name: OTI3A4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 23-241 of human IGSF11 (NP_689751) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: immunoglobulin superfamily member 11
• Database Link:
  NP_689751
  Entrez Gene 207683 MouseEntrez Gene 152404 Human
  UniProt ID Q5DX21
• Background: IGSF11 is an immunoglobulin (Ig) superfamily member that is preferentially expressed in brain and testis. It shares significant homology with coxsackievirus and adenovirus receptor (CXADR; MIM 602621) and endothelial cell-selective adhesion molecule (ESAM). [supplied by OMIM, Apr 2005]
• Synonyms: BT-IgSF; CT119; CXADRL1; Igsf13; VSIG3

Reference Data

• Protein Families: Transmembrane