DDOST Mouse Monoclonal Antibody [Clone ID: OTI1C1]

CAT#: CF803614

Carrier-free (BSA/glycerol-free) DDOST mouse monoclonal antibody, clone OTI1C1 (formerly 1C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDOST (RC200672, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DDOST. Positive lysates LY417440 (100ug) and LC417440 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of A549 cell lysate (35ug) by using anti-DDOST monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-DDOST mouse monoclonal antibody. (TA803614; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Show all 8 images

Specifications

Product Data

• Clone Name: OTI1C1
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 131-378 of human DDOST (NP_005207) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.1 kDa
• Gene Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
• Database Link:
  NP_005207
  Entrez Gene 13200 MouseEntrez Gene 313648 RatEntrez Gene 1650 Human
  UniProt ID P39656
• Background: This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
• Synonyms: AGER1; CDG1R; OKSWcl45; OST; OST48; WBP1

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis