MAP1LC3A Mouse Monoclonal Antibody [Clone ID: OTI10H6]

CAT#: CF803577

Carrier-free (BSA/glycerol-free) MAP1LC3A mouse monoclonal antibody, clone OTI10H6 (formerly 10H6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MAP1LC3A (Cat# RC202222, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAP1LC3A(Cat# TA803577).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MAP1LC3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803577)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MAP1LC3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803577)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MAP1LC3A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA803577)

Specifications

Product Data

• Clone Name: OTI10H6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAP1LC3A (NP_115903) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: microtubule associated protein 1 light chain 3 alpha
• Database Link:
  NP_115903
  Entrez Gene 66734 MouseEntrez Gene 362245 RatEntrez Gene 84557 Human
  UniProt ID Q9H492
• Background: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
• Synonyms: ATG8E; LC3; LC3A; MAP1ALC3; MAP1BLC3