Macro H2A.2 (H2AFY2) Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF803459

Carrier-free (BSA/glycerol-free) H2AFY2 mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY H2AFY2 (RC203699, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-H2AFY2. Positive lysates LY412957 (100ug) and LC412957 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-H2AFY2 mouse monoclonal antibody. (TA803459) Dilution: 1:150

Show all 10 images

Specifications

Product Data

• Clone Name: OTI1F6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-261 of human H2AFY2 (NP_061119) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.9 kDa
• Gene Name: macroH2A.2 histone
• Database Link:
  NP_061119
  Entrez Gene 361844 RatEntrez Gene 404634 MouseEntrez Gene 55506 Human
  UniProt ID Q9P0M6
• Synonyms: macroH2A2

Reference Data

• Protein Pathways: Systemic lupus erythematosus