ZNF500 Mouse Monoclonal Antibody [Clone ID: OTI2D2]

CAT#: CF803348

Carrier-free (BSA/glycerol-free) ZNF500 mouse monoclonal antibody, clone OTI2D2 (formerly 2D2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZNF500 (RC223457, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF500. Positive lysates LY411935 (100ug) and LC411935 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2D2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 102-325 of human ZNF500 (NP_067678) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 53.5 kDa
• Gene Name: Homo sapiens zinc finger protein 500 (ZNF500), transcript variant 1, mRNA.
• Database Link:
  NP_067678
  Entrez Gene 26048 Human
  UniProt ID O60304
• Synonyms: ZKSCAN18; ZSCAN50