PRRX1 Mouse Monoclonal Antibody [Clone ID: OTI6A4]

CAT#: CF803115

Carrier-free (BSA/glycerol-free) PRRX1 mouse monoclonal antibody, clone OTI6A4 (formerly 6A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRRX1 (RC210393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRRX1. Positive lysates LY411607 (100ug) and LC411607 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRRX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA803115)

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Specifications

Product Data

• Clone Name: OTI6A4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRRX1 (NP_073207) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.1 kDa
• Gene Name: paired related homeobox 1
• Database Link:
  NP_073207
  Entrez Gene 18933 MouseEntrez Gene 266813 RatEntrez Gene 5396 Human
  UniProt ID P54821
• Background: The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription co-activator, enhancing the DNA-binding activity of serum response factor, a protein required for the induction of genes by growth and differentiation factors. The protein regulates muscle creatine kinase, indicating a role in the establishment of diverse mesodermal muscle types. Alternative splicing yields two isoforms that differ in abundance and expression patterns. [provided by RefSeq, Jul 2008]
• Synonyms: AGOTC; PHOX1; PMX1; PRX-1; PRX1

Reference Data

• Protein Families: Transcription Factors