HYPE (FICD) Mouse Monoclonal Antibody [Clone ID: OTI2A11]

CAT#: CF802841

Carrier-free (BSA/glycerol-free) FICD mouse monoclonal antibody, clone OTI2A11 (formerly 2A11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FICD (Cat# RC201725, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FICD (Cat# TA802841). Positive lysates LY416213 (100ug) and LC416213 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI2A11
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 61-265 of human FICD (NP_009007) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.6 kDa
• Gene Name: FIC domain protein adenylyltransferase
• Database Link:
  NP_009007
  Entrez Gene 231630 MouseEntrez Gene 288741 RatEntrez Gene 11153 Human
  UniProt ID Q9BVA6
• Synonyms: HIP13; HYPE; UNQ3041

Reference Data

• Protein Families: Transmembrane