AFAP (AFAP1) Mouse Monoclonal Antibody [Clone ID: OTI5G2]

CAT#: CF802330

Carrier-free (BSA/glycerol-free) AFAP1 mouse monoclonal antibody, clone OTI5G2 (formerly 5G2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFAP1 (RC215587, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFAP1. Positive lysates LY404799 (100ug) and LC404799 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI5G2
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 100-386 of human AFAP1 (NP_940997) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 80.5 kDa
• Gene Name: actin filament associated protein 1
• Database Link:
  NP_940997
  Entrez Gene 70292 MouseEntrez Gene 140935 RatEntrez Gene 60312 Human
  UniProt ID Q8N556
• Background: The protein encoded by this gene is a Src binding partner. It may represent a potential modulator of actin filament integrity in response to cellular signals, and may function as an adaptor protein by linking Src family members and/or other signaling proteins to actin filaments. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Synonyms: AFAP; AFAP-110; AFAP110