VILIP1 (VSNL1) Mouse Monoclonal Antibody [Clone ID: OTI3A1]

CAT#: CF802258

Carrier-free (BSA/glycerol-free) VSNL1 mouse monoclonal antibody,clone OTI3A1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VSNL1 (RC205337, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VSNL1. Positive lysates LY418720 (100ug) and LC418720 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI3A1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-191 of human VSNL1 (NP_003376) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22 kDa
• Gene Name: Homo sapiens visinin like 1 (VSNL1), transcript variant 1, mRNA.
• Database Link:
  NP_003376
  Entrez Gene 24877 RatEntrez Gene 26950 MouseEntrez Gene 7447 Human
  UniProt ID P62760
• Background: This gene is a member of the visinin/recoverin subfamily of neuronal calcium sensor proteins. The encoded protein is strongly expressed in granule cells of the cerebellum where it associates with membranes in a calcium-dependent manner and modulates intracellular signaling pathways of the central nervous system by directly or indirectly regulating the activity of adenylyl cyclase. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: HLP3; HPCAL3; HUVISL1; VILIP; VILIP-1

Reference Data

• Protein Families: Druggable Genome