Argininosuccinate Lyase (ASL) Mouse Monoclonal Antibody [Clone ID: OTI3D4]

CAT#: CF802245

Carrier-free (BSA/glycerol-free) ASL mouse monoclonal antibody, clone OTI3D4 (formerly 3D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ASL (Cat# RC201568, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASL(Cat# TA802245). Positive lysates LY422562 (100ug) and LC422562 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from different cell line by using anti-ASL monoclonal antibody (1:500).

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  Western blot analysis of HT29 cell lysate (35ug) by using anti-ASL monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ASL mouse monoclonal antibody. (TA802245) Dilution: 1:150

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ASL mouse monoclonal antibody. (TA802245) Dilution: 1:150

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Specifications

Product Data

• Clone Name: OTI3D4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ASL (NP_001020114) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51.5 kDa
• Gene Name: argininosuccinate lyase
• Database Link:
  NP_001020114
  Entrez Gene 59085 RatEntrez Gene 109900 MouseEntrez Gene 435 Human
  UniProt ID P04424
• Background: This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul
• Synonyms: ASAL

Reference Data

• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Metabolic pathways