OXSM Mouse Monoclonal Antibody [Clone ID: OTI1G5]

CAT#: CF802187

Carrier-free (BSA/glycerol-free) OXSM mouse monoclonal antibody, clone OTI1G5 (formerly 1G5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OXSM (RC201500, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OXSM. Positive lysates LY413479 (100ug) and LC413479 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OXSM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802187)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-OXSM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802187)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-OXSM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802187)

Specifications

Product Data

• Clone Name: OTI1G5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 78-343 of human OXSM (NP_060367) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.7 kDa
• Gene Name: 3-oxoacyl-ACP synthase, mitochondrial
• Database Link:
  NP_060367
  Entrez Gene 71147 MouseEntrez Gene 289934 RatEntrez Gene 54995 Human
  UniProt ID Q9NWU1
• Background: This gene encodes a beta-ketoacyl synthetase. The encoded enzyme is required for elongation of fatty acid chains in the mitochondria. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
• Synonyms: FASN2D; KASI; KS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid biosynthesis, Metabolic pathways