NUMB Mouse Monoclonal Antibody [Clone ID: OTI3E5]

CAT#: CF802169

Carrier-free (BSA/glycerol-free) NUMB mouse monoclonal antibody, clone OTI3E5 (formerly 3E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC221018, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB. Positive lysates LY423644 (100ug) and LC423644 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802169)

• 

  Immunohistochemical staining of paraffin-embedded Human muscle tissue using anti-NUMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802169)

Specifications

Product Data

• Clone Name: OTI3E5
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-365 of human NUMB (NP_001005744) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 65.7 kDa
• Gene Name: Homo sapiens NUMB endocytic adaptor protein (NUMB), transcript variant 2, mRNA.
• Database Link:
  NP_001005744
  Entrez Gene 18222 MouseEntrez Gene 29419 RatEntrez Gene 8650 Human
  UniProt ID P49757
• Background: The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: C14orf41; c14_5527; S171

Reference Data

• Protein Pathways: Notch signaling pathway