Smad Interacting Protein 1 (ZEB2) Mouse Monoclonal Antibody [Clone ID: OTI1E12]

CAT#: CF802113

Carrier-free (BSA/glycerol-free) ZEB2 mouse monoclonal antibody,clone OTI1E12

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZEB2 (Cat# RC215227, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZEB2(Cat# TA802113).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ZEB2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802113)

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Specifications

Product Data

• Clone Name: OTI1E12
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-350 of human ZEB2 (NP_055610) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens zinc finger E-box binding homeobox 2 (ZEB2), transcript variant 1, mRNA.
• Database Link:
  NP_055610
  Entrez Gene 24136 MouseEntrez Gene 311071 RatEntrez Gene 9839 Human
  UniProt ID O60315
• Background: The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2010]
• Synonyms: HSPC082; SIP-1; SIP1; SMADIP1; ZFHX1B

Reference Data

• Protein Families: Druggable Genome, Transcription Factors