PI 3 Kinase Class 2A (PIK3C2A) Mouse Monoclonal Antibody [Clone ID: OTI3H2]

CAT#: CF801692

Carrier-free (BSA/glycerol-free) PIK3C2A mouse monoclonal antibody, clone OTI3H2 (formerly 3H2)

Product Images

E.coli lysate (left lane) and E.coli lysate expressing human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) were separated by SDS-PAGE and immunoblotted with anti-PIK3C2A.

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  Western blot analysis of HT29 cell lysate (35ug) by using anti-PIK3C2A monoclonal antibody. Dilution: 1:500

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PIK3C2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA801692)

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Specifications

Product Data

• Clone Name: OTI3H2
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 230-560 of human PIK3C2A (NP_002636) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 190.5 kDa
• Gene Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
• Database Link:
  NP_002636
  Entrez Gene 18704 MouseEntrez Gene 5286 Human
  UniProt ID O00443
• Synonyms: CPK; PI3-K-C2(ALPHA); PI3-K-C2A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system