E Cadherin (CDH1) Mouse Monoclonal Antibody [Clone ID: OTI2D6]

CAT#: CF800669

Carrier-free (BSA/glycerol-free) CDH1 mouse monoclonal antibody, clone OTI2D6 (formerly 2D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH1 (RC220731, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH1 (1:500).

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  Western blot analysis of extracts (35ug) from 8 cell lines lysates by using anti-CDH1 monoclonal antibody (1:500).

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  Western blot analysis of extracts (35ug) from 2 tissue lysates by using anti-CDH1 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CDH1mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

• Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

• Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-CDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA800669) (1:500)

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Specifications

Product Data

• Clone Name: OTI2D6
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDH1 (NP_004351) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 94.8 kDa
• Gene Name: cadherin 1
• Database Link:
  NP_004351
  Entrez Gene 999 Human
  UniProt ID P12830
• Background: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq, Jul 2008]
• Synonyms: Arc-1; CD324; CDHE; ECAD; LCAM; UVO

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Adherens junction, Bladder cancer, Cell adhesion molecules (CAMs), Endometrial cancer, Melanoma, Pathogenic Escherichia coli infection, Pathways in cancer, Thyroid cancer