A4GNT Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CAT#: CF800432

Carrier-free (BSA/glycerol-free) A4GNT mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY A4GNT (RC221256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-A4GNT. Positive lysates LY414153 (100ug) and LC414153 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 121-340 of human A4GNT (NP_057245) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.3 kDa
• Gene Name: alpha-1,4-N-acetylglucosaminyltransferase
• Database Link:
  NP_057245
  Entrez Gene 51146 Human
  UniProt ID Q9UNA3
• Background: This gene encodes a protein from the glycosyltransferase 32 family. The enzyme catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. It forms a unique glycan, GlcNAcalpha1-->4Galbeta-->R and is largely associated with the Golgi apparatus membrane. [provided by RefSeq]
• Synonyms: alpha4GnT

Reference Data

• Protein Families: Transmembrane