Isocitrate dehydrogenase (IDH1) Mouse Monoclonal Antibody [Clone ID: OTI3G9]

CAT#: CF800406

Carrier-free (BSA/glycerol-free) IDH1 mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH1 (RC210582, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH1. Positive lysates LY401782 (100ug) and LC401782 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  HEK293T cells were either not tranfected (left lane "293T") or transfected with pCMV6-ENTRY IDH1 (wild type-SKU# RC210582, middle lane "WT") or pCMV6-ENTRY IDH1 mutated (R132H mutation-SKU# RC400096, right lane "R132H") cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (10 ug per lane) were separated by SDS-PAGE and immunoblotted with TA800406 (1:500) and then goat anti-mouse IgG-HRP (1:2000).

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and IDH1-Knockout Hela cells (KO, Cat# LC810112) were separated by SDS-PAGE and immunoblotted with anti-IDH1 monoclonal antibody TA800406. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

• 

  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800406)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IDH1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800406)

Show all 6 images

Specifications

Product Data

• Clone Name: OTI3G9
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Dog, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide around the R132H mutation region of the human IDH conjugated to KLH
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46.5 kDa
• Gene Name: Homo sapiens isocitrate dehydrogenase (NADP(+)) 1 (IDH1), transcript variant 1, mRNA.
• Database Link:
  NP_005887
  Entrez Gene 15926 MouseEntrez Gene 24479 RatEntrez Gene 478889 DogEntrez Gene 3417 Human
  UniProt ID O75874
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq, Jul 2008]
• Synonyms: HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways