FBXO42 Mouse Monoclonal Antibody [Clone ID: OTI1H4]

CAT#: CF800283

Carrier-free (BSA/glycerol-free) FBXO42 mouse monoclonal antibody, clone OTI1H4 (formerly 1H4)

Product Images

HEK293T cells transfected with either RC209600 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FBXO42 antibody (TA800283), and then analyzed by flow cytometry.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FBXO42 (RC209600, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FBXO42. Positive lysates LY412832 (100ug) and LC412832 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800283)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800283)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800283)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FBXO42 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800283)

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Specifications

Product Data

• Clone Name: OTI1H4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 356-717 of human FBXO42 (NP_061867) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 77.7 kDa
• Gene Name: F-box protein 42
• Database Link:
  NP_061867
  Entrez Gene 213499 MouseEntrez Gene 362646 RatEntrez Gene 54455 Human
  UniProt ID Q6P3S6
• Background: Members of the F-box protein family, such as FBXO42, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (SKP1A; MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]). [supplied by OMIM]
• Synonyms: Fbx42; JFK

Reference Data

• Protein Families: Druggable Genome