FOXI1 Mouse Monoclonal Antibody [Clone ID: OTI2B8]

CAT#: CF800145

Carrier-free (BSA/glycerol-free) FOXI1 mouse monoclonal antibody, clone OTI2B8 (formerly 2B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOXI1 (RC218102, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOXI1. Positive lysates LY402162 (100ug) and LC402162 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FOXI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800145)

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  Anti-FOXI1 mouse monoclonal antibody (TA800145) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FOXI1 (RC218102).

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  HEK293T cells transfected with either RC218102 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOXI1 antibody (TA800145), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2B8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 111-350 of human FOXI1 (NP_036320) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.8 kDa
• Gene Name: forkhead box I1
• Database Link:
  NP_036320
  Entrez Gene 14233 MouseEntrez Gene 2299 Human
  UniProt ID Q12951
• Background: This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it is possible that this gene plays an important role in the development of the cochlea and vestibulum, as well as embryogenesis. Mutations in this gene may be associated with the common cavity phenotype. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: FKH10; FKHL10; FREAC-6; FREAC6; HFH-3; HFH3

Reference Data

• Protein Families: Transcription Factors