SENP2 Mouse Monoclonal Antibody [Clone ID: OTI5G8]

CAT#: CF800126

Carrier-free (BSA/glycerol-free) SENP2 mouse monoclonal antibody, clone OTI5G8 (formerly 5G8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SENP2 (RC208109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SENP2.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SENP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SENP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800126)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SENP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800126)

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  HEK293T cells transfected with either RC208109 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SENP2 antibody (TA800126), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5G8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 139-523 of human SENP2 (NP_067640) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Homo sapiens SUMO specific peptidase 2 (SENP2), mRNA.
• Database Link:
  NP_067640
  Entrez Gene 75826 MouseEntrez Gene 78973 RatEntrez Gene 59343 Human
  UniProt ID Q9HC62
• Background: SUMO1 (UBL1; MIM 601912) is a small ubiquitin-like protein that can be covalently conjugated to other proteins. SENP2 is one of a group of enzymes that process newly synthesized SUMO1 into the conjugatable form and catalyze the deconjugation of SUMO1-containing species. [supplied by OMIM]. COMPLETENESS: complete on the 3' end.
• Synonyms: AXAM2; SMT3IP2

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Wnt signaling pathway