CNOT4 Mouse Monoclonal Antibody [Clone ID: OTI1F10]

CAT#: CF800048

Carrier-free (glycerol/BSA-free) CNOT4 mouse monoclonal antibody, clone OTI1F10 (formerly 1F10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CNOT4 (RC217418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CNOT4. Positive lysates LY415669 (100ug) and LC415669 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-CNOT4 Mouse monoclonal antibody. (TA800048; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-CNOT4 Mouse monoclonal antibody. (TA800048; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Specifications

Product Data

• Clone Name: OTI1F10
• Applications: IHC, WB
• Recommended Dilution: IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 190-455 of human CNOT4 (NP_037448) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.2 kDa
• Gene Name: CCR4-NOT transcription complex subunit 4
• Database Link:
  NP_037448
  Entrez Gene 53621 MouseEntrez Gene 312227 RatEntrez Gene 4850 Human
  UniProt ID O95628
• Background: The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
• Synonyms: CLONE243; NOT4; NOT4H

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RNA degradation