SENP1 Mouse Monoclonal Antibody [Clone ID: OTI1D7]

CAT#: CF800001

Carrier-free (BSA/glycerol-free) SENP1 mouse monoclonal antibody, clone OTI1D7 (formerly 1D7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SENP1 (Cat# [RC207194], Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SENP1(Cat# TA800001).

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  HEK293T cells transfected with either [RC207194] overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SENP1 antibody (TA800001), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1D7
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 221-460 of human SENP1 (NP_055369) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73.2 kDa
• Gene Name: Homo sapiens SUMO1/sentrin specific peptidase 1 (SENP1)
• Database Link:
  NP_055369
  Entrez Gene 223870 MouseEntrez Gene 29843 Human
  UniProt ID Q9P0U3
• Background: The covalent modification of proteins by the small ubiquitin (UBB; MIM 191339)-like protein SUMO (see SUMO1, MIM 601912) is implicated in the regulation of nucleocytoplasmic transport, genomic stability, gene transcription, and other processes. Sumoylation is catalyzed on target lysine residues by a multienzyme process and is reversed by desumoylating enzymes such as SENP1 (Yamaguchi et al., 2005 [PubMed 15923632]). [supplied by OMIM]
• Synonyms: SuPr-2

Reference Data

• Protein Families: Druggable Genome, Protease