Sex Hormone Binding Globulin (SHBG) Mouse Monoclonal Antibody [Clone ID: OTI1D10]

CAT#: CF507136

Carrier-free (BSA/glycerol-free) SHBG mouse monoclonal antibody, clone OTI1D10 (formerly 1D10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHBG (RC228307, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHBG. Positive lysates LY431335 (100ug) and LC431335 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-SHBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA507136)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SHBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA507136)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SHBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA507136)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SHBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA507136)

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Specifications

Product Data

• Clone Name: OTI1D10
• Applications: IHC, WB
• Recommended Dilution: WB 1:4000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SHBG(NP_001139751) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.4 kDa
• Gene Name: Homo sapiens sex hormone binding globulin (SHBG), transcript variant 2, mRNA.
• Database Link:
  NP_001139751
  Entrez Gene 6462 Human
  UniProt ID P04278
• Background: This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein binds each steroid molecule as a dimer formed from identical or nearly identical monomers. The use of alternate promoters and alternatively spliced transcripts have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
• Synonyms: ABP; SBP; TEBG

Reference Data

• Protein Families: Druggable Genome, Secreted Protein