MT (MCAT) Mouse Monoclonal Antibody [Clone ID: OTI2G1]

CAT#: CF507071

Carrier-free (BSA/glycerol-free) MCAT mouse monoclonal antibody, clone OTI2G1 (formerly 2G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCAT (RC207943, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCAT. Positive lysates LY406620 (100ug) and LC406620 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MCAT monoclonal antibody at 1:400 dilution (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCAT (RC207943) using Anti-MCAT mouse monoclonal antibody (TA507071) at 1:100 dilution.

Specifications

Product Data

• Clone Name: OTI2G1
• Applications: IF, WB
• Recommended Dilution: WB 1:1000 -1:4000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MCAT(NP_775738) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.6 kDa
• Gene Name: malonyl-CoA-acyl carrier protein transacylase
• Database Link:
  NP_775738
  Entrez Gene 27349 Human
  UniProt ID Q8IVS2
• Background: The protein encoded by this gene is found exclusively in the mitochondrion, where it catalyzes the transfer of a malonyl group from malonyl-CoA to the mitochondrial acyl carrier protein. The encoded protein may be part of a fatty acid synthase complex that is more like the type II prokaryotic and plastid complexes rather than the type I human cytosolic complex. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2012]
• Synonyms: fabD; FASN2C; MCT; MT; NET62

Reference Data

• Protein Pathways: Fatty acid biosynthesis, Metabolic pathways